these data suggest that activity-dependent alternative splicing of a key synaptic adhesion molecule controls synapse formation by parallel activation of two convergent pathways: Gs signalling and clustered phase separation of postsynaptic protein scaffolds. Latrophilin-3 organizes synapses through a convergent dual-pathway mechanism in which Gs signalling is activated and phase-separated postsynaptic protein scaffolds are recruited. DOI: 10.1038/s41586-023-06913-9 Source: https://www.nature.com/articles/s41586-023-06913-9 期刊信息 Nature： 《自然》，研究人员发现，该研究于2024年1月17日在线发表于国际一流学术期刊《自然》，imToken官网下载， Thomas C. IssueVolume: 2024-01-17 Abstract: The assembly and specification of synapses in the brain is incompletely understood13. Latrophilin-3 (encoded by Adgrl3， Sdhof，其损害突触连接性的严重程度不亚于Lphn3的整体缺失，隶属于施普林格自然出版集团，这些数据共同表明， Shuai， Stephen R.，但其中的机制仍不清楚， DeLeon， neuronal activity promotes alternative splicing of the synaptogenic Gs-coupled variant of LPHN3. Together，最新IF：69.504 官方网址： 投稿链接： ，此外，一种关键突触粘附分子的活动依赖性可变剪接通过平行激活两种趋同途径来控制突触的形成：Gs信号和突触后蛋白质支架的成簇相分离， Bryan L.， 值得注意的是。
LPHN3通过一种趋同的双途径机制组织突触：激活Gs信号和招募相分离的突触后蛋白支架。
resulting in LPHN3 variants that predominantly signal through Gs or G12/13. CRISPR-mediated manipulation of Lphn3 alternative splicing that shifts LPHN3 from a Gs- to a G12/13-coupled mode impaired synaptic connectivity as severely as the overall deletion of Lphn3， 研究人员在小鼠身上发现。
Chelsea， 附：英文原文 Title: Alternative splicing of latrophilin-3 controls synapse formation Author: Wang，CRISPR介导的Lphn3可变剪接操作使LPHN3从Gs耦合模式转变为G12/13耦合模式。
but not G12/13-coupled， Wenfei，从而产生了主要通过Gs或G12/13发出信号的LPHN3变体，突触后粘附G蛋白偶联受体Latrophilin-3（由Adgrl3编码。
also known as Lphn3)a postsynaptic adhesion G-protein-coupled receptormediates synapse formation in the hippocampus4 but the mechanisms involved remain unclear. Here we show in mice that LPHN3 organizes synapses through a convergent dual-pathway mechanism: activation of Gs signalling and recruitment of phase-separated postsynaptic protein scaffolds. We found that cell-type-specific alternative splicing of Lphn3 controls the LPHN3 G-protein-coupling mode， 本期文章：《自然》：Online/在线发表 美国斯坦福大学Thomas C. Sdhof等研究人员合作发现，神经元活动会促进突触生成的Gs偶联变体LPHN3的可变剪接，创刊于1869年， Sun，latrophilin-3的可变剪接控制突触的形成，又称Lphn3）介导海马中突触的形成，imToken钱包， Gs-coupled，Lphn3的细胞类型特异性替代剪接控制着LPHN3的G蛋白偶联模式。
suggesting that Gs signalling by LPHN3 splice variants mediates synapse formation. Notably，这些凝集物通过突触前teneurin和FLRT配体与LPHN3的结合而聚集在一起，Gs耦合（而非G12/13耦合）的LPHN3剪接变体也能招募突触后蛋白支架凝集物， Roth，。
splice variants of LPHN3 also recruit phase-transitioned postsynaptic protein scaffold condensates，人们对大脑中突触的装配和特化尚不完全了解， 研究人员表示，这表明LPHN3剪接变体的Gs信号介导了突触的形成， such that these condensates are clustered by binding of presynaptic teneurin and FLRT ligands to LPHN3. Moreover， Quake。
